Researcher

Matthew Federici

Objective

Test the solubility of 4-bromophenylacetic acid, 4-hydroxybenzaldehyde, 2,4,6-trimethoxybenzaldehyde, 2,6 dichlorophenylacetic acid, 4-nitrobenzaldehyde, 3,4-(methylenedioxy)phenylacetic acid, phenylpropiolic acid, and N-(tert-butoxycarbonyl)-L serine in methanol, acetonitrile, and DMSO.

Results

Spreadsheet[DOSOL was removed from the experiment list but why are there unfinished rows still? JCB]

Discussion


Conclusion


Log


5-21-2009

Vial Label
Solute
A
4-bromophenylacetic acid
B
4-hydroxybenzaldehyde
C
2,4,6-trimethoxybenzaldehyde
D
2,6 dichlorophenylacetic acid
E
4-nitrobenzaldehyde
F
3,4-(methylenedioxy)phenylacetic acid
G
phenylpropiolic acid
H
N-(tert-butoxycarbonyl)-L serine


17:58 Three half dram vials were labeled A1, A2, and A3. 250uL of methanol was pippeted into vial A1. 4-bromophenylacetic acid was added to vial A1. The vial was vortexed for 20 seconds. After several trials of adding 4-bromophenylacetic acid and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials A2 and A3 wtih acetonitrile and DMSO respectively.


18:11 Three half dram vials were labeled B1, B2, and B3. 250uL of methanol was pippeted into vial B1. 4-hydroxybenzaldehyde was added to vial B1. The vial was vortexed for 20 seconds. After several trials of adding 4-hydroxybenzaldehyde and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials B2 and B3 wtih acetonitrile and DMSO respectively. Too much 4-hydroxybenzaldehyde was added to vial B2 so 250 more uL was added to that vial. After vortexing for 20 seconds, the 500uL solution appeared to be saturated.

18:36 Three half dram vials were labeled C1, C2, and C3. 250uL of methanol was pippeted into vial C1. 2,4,6-trimethoxybenzaldehyde
was added to vial C1. The vial was vortexed for 20 seconds. After several trials of adding 2,4,6-trimethoxybenzaldehyde
and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials C2 and C3 wtih acetonitrile and DMSO respectively.

18:45 Three half dram vials were labeled D1, D2, and D3. 250uL of methanol was pippeted into vial D1. 2,6 dichlorophenylacetic acid
was added to vial D1. Too much 2,6 dichlorophenylacetic acid was added so 250uL of methanol was added to the solution. The vial was vortexed for 20 seconds. The solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. A 250uL saturated solution of acetonitrile and 2,6 dichlorophenylacetic acid
was created in vial D2. A 500 uL saturated solution of DMSO and 2,6 dichlorophenylacetic acid was created in vial D3.

18:54 Vials A1, A2, A3, B1, B2, B3, C1, C2, C3, D1, D2, and D3 were placed in a small beaker with some water. The beaker was placed in a sonicator. The bath temperature was 30C. The sonicator was turned on and set to 30 minutes.

18:57 Three half dram vials were labeled E1, E2, and E3. 250uL of methanol was pippeted into vial E1. 4-nitrobenzaldehyde was added to vial E1. Too much 4-nitrobenzaldehyde was added into the vial so an additional 250uL of of methanol was added. The vial was vortexed for 20 seconds. After vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials A2 and A3 wtih 250 uL of acetonitrile and 250 uL of DMSO respectively. The DMSO solution was a dark purple color. Usually, 4-nitrobenzaldehyde creates light colored solutions. The methanol solution and acetonitrile solution were light tan.

19:04 The lid of C1 and B1 came off in the sonicator. C1 tipped into the beaker of water. The vial was discarded. A new cap was added to B1. It was noticed that A3 did not have any solid at the bottom of the vial. This vial was removed from sonication. More 4-bromophenyacetic acid was added to this vial. The vial was sonicated and left to be sonicated with the next group of vials. During this process, sonication was not stopped. All other vials remained in the beaker of water and sonication bath.

19:15 Three half dram vials were labeled F1, F2, and F3. 250uL of methanol was pippeted into vial F1. 3,4-(methylenedioxy)phenylacetic acid was added to vial F1. The vial was vortexed for 20 seconds. After several trials of adding 3,4-(methylenedioxy)phenylacetic acid and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials F2 and F3 wtih acetonitrile and DMSO respectively.

19:27 The beaker was taken out of the sonicator. The cap from B1 had fallen off and spilled into the beaker. This vial was discarded. There was no solid in A1 or A2. The bath temperature was 42C.

19:29 Three half dram vials were labeled G1, G2, and G3. 250uL of methanol was pippeted into vial G1. Phenylpropiolic acid was added to vial G1. The vial was vortexed for 20 seconds. After several trials of adding phenypropiolic acid and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials G2 and G3 wtih acetonitrile and DMSO respectively.

19:36 Three half dram vials were labeled H1, H2, and H3. 250uL of methanol was pippeted into vial H1. N-(tert-butoxycarbonyl)-L serine was added to vial H1. The vial was vortexed for 20 seconds. After several trials of adding N-(tert-butoxycarbonyl)-L serine and vortexing for 20 seconds, the solution appeared to be saturated. The solution appeared cloudy and solid was forming at the bottom. After several minutes (before sonication), solid formed at the bottom and the solution appeared to be clear. This process was repeated for vials H2 and H3 wtih acetonitrile and DMSO respectively.

19:46 Vials A3, E1, E2, E3, F1, F2, F3, G1, G2, G3, H1, H2, and H3 were placed in a beaker with some water. The beaker was placed in a sonicator. The sonicator was turned on and set to 30 minutes. The bath temperature was 35C.

20:15 All vials were removed from the sonicator. There was no solid in A3, H1, or G3. The lid from vial F1 had popped off but it did not spill in the beaker. All vials were left on the workbench. [would be a good idea also to record ambient temp at the bench --BH][Even better to let equilibrate in a water bath measured to be 22C JCB]