Exp075

media type="custom" key="3902969"

Researchers
Shuba Dumpala, Khalid Mirza

Objective
To determine the solubility of 4-chlorophenylacetic acid in acetonitrile, toluene, THF and DMSO.

Results
[|ONSCExp075-1]  [|ONSCExp075-3]  [|ONSCExp075-4] [|ONSCExp075-5] [|ONSCExp075-6] [|Spreadsheet-2] media type="custom" key="3902985"
 * HNMR**
 * [Add column with links to all the raw JCAMP-DX files AL]**

Discussion
While perfoming the NMR, there were some magnetic disturbances, since there were distortions in the peaks.

Conclusion
In the NMR test, the peaks were a little broad near the baseline. Hence, they were reprocessed in order to determine the solubility.

2009-04-09
12:45 Labeled seven half-dram vial from 1-7. 12:50 Placed a small amount of 4-chlorophenylacetic acid in each vial. 13:00 Pipetted out 200uL of seven solvents in the vials listed below. After adding the solvent to the vials, each was vortexed for about 30s. 13:10 Placed the vials in a 100mL beaker containing a small amount of water, this beaker was left in the sonicator. 13:20 Temperature of the water in the sonicator was recorded as 30 C. 13:30 The vials were observed for crystals. 16:05 Two of the vials with Methanol and acetronitrile had to be repeated for better results. 11:46 Obtained a picture of the saturated solutions after sonication - **ONSCExp075-1** 12:15 The vials with methanol and acetonitrile were refilled with diphenyl acetic acid this time because the 4-chlorophenyl acetic acid was exhausted. These solutions have therefore been discarded.**[if they were discarded why are there results for acetonitrile? JCB]** 12:25 The vials were then placed in the sonicator 12:35 The temperature of the water in the sonicator was observed to be 28 C. 12:45 The vials were removed from the sonicator. 12:50 The vials were centrifuged. 13:00 The supernatents were collected into clean dry test-tubes from the vials by adding 0.71uM Chloroform into it. 11:50 Test-tubes 3,4,5, and 6 were tested with NMR
 * Vial# || Solvent ||
 * 1 || methanol ||
 * 2 || ethanol ||
 * 3 || acetonitrile ||
 * 4 || toluene ||
 * 5 || THF ||
 * 6 || DMSO ||
 * 7 || chloroform ||
 * 2009-04-10**
 * 2009-04-11**