Exp154

=Researchers=
 * Hai Truong and Khalid Mirza**

=Objective= To study the effect of phenylacetic acid on the solubility of the Ugi product UCExp17C at lower concentration (0.01M - 0.15) in benzene

=Procedure= 154C - 0.1M - Pipette out 200uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene 154D - 0.075M - Pipette out 150uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene 154E - 0.05M - Pipette out 100uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene 154F - 0.03M - Pipette out 60uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene 154G - 0.01M- Pipette out 20uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene =Results= [|Spreadsheet ONSCExp154-i] media type="googlespreadsheet" key="toOGE0XTSJXQiTshp1kL9_A" height="500" width="800" =Discussion= see plot
 * 154A**- **0.5M**: Dissolve phenylacetic acid (1mmol, 136mg) in benzene in a 2mL volumetric flask - Stock solution
 * 154B** - 0.2M - Pipette out 400uL of 154A in to a 1mL volumetric flask and make it up to the mark with benzene

=Conclusion=

= = =Log= 18:15 Weighed out 136mg of phenylacetic acid and put into a 2ml volumetric flask. Filled up to the mark with benzene. This is the stock solution-**154A-0.5M** 18:20 Transferred stock solution into a dram vial to store it in the refrigerator to prevent evaporation. 11:10 Changed the temperature setting of the water-glycol to 25C. 17:15 Put the stock solution in 25C water for 20 minutes. 17:35 Took out six 1ml-volumetric flask from the hot oven. Labeled B,C,D,E,F, and G. In volumetric flask B: pipetted out 400uL of 154A into flask B and make it up to the mark. This solution is 0.2M In volumetric flask C: pipetted out 200uL of 154A into flask C and make it up to the mark. This solution is 0.1M In volumetric flask D: pipetted out 150uL of 154A into flask D and make it up to the mark. This solution is 0.075M In volumetric flask E: pipetted out 100uL of 154A into flask E and make it up to the mark. This solution is 0.05M In volumetric flask F: pipetted out 60uL of 154A into flask F and make it up to the mark. This solution is 0.03M In volumetric flask G: pipetted out 20uL of 154A into flask G and make it up to the mark. This solution is 0.01M 17:45 Took out 7 half dram vials. Labeled **Exp154-A, 154-B, 154-C, 154-D, 154-E, 154-F,** and **154-G**. In vial Exp154-A: pipetted out 500uL out of stock solution 154-A into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-B: pipetted out 500uL out of flask B into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-C: pipetted out 500uL out of flask C into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-D: pipetted out 500uL out of flask D into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-E: pipetted out 500uL out of flask E into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-F: pipetted out 500uL out of flask F into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. In vial Exp154-G: pipetted out 500uL out of flask G into this vial. Put small amount of Ugi176C so that there was still small amount of solid remain in the solution. 18:10 Parafilmed all the vials 18:30 Put all vial into the Lauda heating/cooling bath. The temperature in the bath was 50C. 19:35 Adjusted temperature in the bath to 40C. 17:55 Changed the temperature setting on the bath from 40C to 25C. Checked and made sure all the sample vials appeared ok-no leak! 11:20 Removed the vials from the temperature control bath. All vials contained solvent along with solid in them. 11:30 Centrifuged the vial. 11:35 Pipetted out 400uL DMSO-d6 in to four clean and dry MR tubes. Labeled them ONSCExp154-A-1 through ONSCExo154-G-1. 11:55 Carefully pipetted out approximately 200uL supernatant solution from each vial and transferred it in to a corresponding NMR tube. 14:45 Obtained HNMR of the solution- HNMRs labeled ONSCExp154-A-1 to ONSCExp154-G-1.
 * 12-04-2009**
 * [this won't be an issue for benzene boiling at 80C JCB]- [ok - Hai]**
 * 12-05-2009**
 * 12-11-2009**
 * 12-13-2009**
 * 12-15-2009**