Exp145

media type="custom" key="4718985" =Researcher=

Hai & Khalid
=Objective= To determine the solubility of [|UCExp251-2C] from UCExp251 (UC148B) in a) Benzene, b) THF, c) acetonitrile, d) methanol and e) ethanol = = =Procedure= To five labeled half dram vials, a small amount of UCExp251-2C was added to each vials containg a) Benzene, b) THF, c) acetonitrile, d) methanol and e) ethanol. Put them into Lauda heating/cooling device at 40C in half an hour, and cool them dowm at 25C in half an hour. Proton HNMR spectra of supernatant from each solution was obtained on a 500MHz Varian invo instrument. Solubility was determined by using [|SAMS]. =Result=

[|SAMS Solubility calculation Spreadsheet]

=Discussion= =Conclusion=

[|Link] to the solubiltiy of 148B in different organic solvents.

=Log= 17:40 Turned on a "Lauda heating/cooling" device. Set up temperature to exact 40C 17:45 Took out 5 half-dram vials, labeled **ONSC145-1A**, **ONSC145-2A**, **ONSC145-3A**, **ONSC145-4A** and **ONSC145-5A**. Put 200ul of benzene into vial 1, THF into vial 2, acetonitrile into vial 3, methanol into vial 4, ethanol into vial 5. 17:50 Took out small mount of solid  [|UCExp251-2C] into each vial. Vortexed each vials carefully to make sure there is still solid remained in each vials. Parafilmed carefully all the vials. Took a photo of 5 vials before putting into the bath. 17:55 Put 5 vials into "Lauda heating/cooling" device. At this time, the temperature in the bath was 40C. 18: 25 Lowered the bath temperature to 25C. 18:55 The temperature in the bath reached 25C 11:20 Removed the samples from the 25C bath and centrifuged the vials. 11:30 Pipetted out 700uL CDCl3 in to five labled one dram vials 11:40 Carefully pipetted out 100uL of the supernatants Supernatant from the half dram vial ONSCExp145-1A in to the one dam vial**; ONSCExp145-1B** Supernatant from the half dram vial ONSCExp145-2A in to the one dam vial**; ONSCExp145-2B** Supernatant from the half dram vial ONSCExp145-3A in to the one dam vial**; ONSCExp145-3B,** Supernatant from the half dram vial ONSCExp145-4A in to the one dam vial**; ONSCExp145-4B** Supernatant from the half dram vial ONSCExp145-5A in to the one dam vial**; ONSCExp145-5B**, vortexed the resulting solution and placed them in to five clean and dry NMR tubes. 17:40 Obtained HNMRs of **ONSCExp145-1B,** **ONSCExp145-2B,** **ONSCExp145-3B,** **ONSCExp145-4B** and **ONSCExp145-5B**.
 * 2009-11-03**
 * 2009-11-04**