Exp057


 * [This experiment number is already taken - make sure to add to the [|experiment list] as soon as you create an experiment to avoid this JCB]**

=Log=

2009-02-09
16:17 Sterilzed Erlenmeyer flasks, spatulas, and thermometers with the use of acetone, and briefly placed them in the laboratory oven to completely evaporate off the acetone. 16:26 Placed 3-4mL of ethanol into a 25mL Erlenmeyer flask. The same was done for chloroform. 16:28 Placed flasks into an ice-bath. 16:30 Measured ambient room temperature to be 32ºC.**[That can't be right JCB]** 16:33 Filled vial with 600 μl of room temperature ethanol. Labeled vial accordingly.**[What is the label? 57-1? JCB]** 16:34 Filled a vial with 600 μl of room temperature chloroform. Labeled vial accordingly.**[What is the label? JCB]** 16:36 Added 1-pyrenebutyric acid solute to vial containing room temperature ethanol. 16:37 Added 1-pyrenebutyric acid solute to vial containing room temperature chloroform. 16:39 Vortexed both vials for 10 minutes. Vials had a yellow-to-goldish look to them. 16:50 Added 600 μl of respective solvent to their respective vials. 16:52 Vortexed both vials for another 10 minutes. 17:07 Centrifuged the vials for 5 minutes. 17:12 Left vials to equilibriate over night. H3C-OH @ 32ºC, CHCl3 @ 32ºC 17:35 Measured temperature of the Erlenmeyer flasks containing ethanol and chloroform using a thermometer, but the desired temperature had not beend reached. Therefore, the vials where placed in a bath of dry-ice. 17:50 Once the temperature of the ethanol and chloroform reached -10ºC, 600 μl of ethanol was pipetted into a vial and 600 μl of chloroform was pipetted into another vial. Both vials were labeled accordingly. 17:52 Added 1-pyrenebutyric acid to each vial. 17:52 Vortexed the vials for 10 minutes. 18:02 Centrifuged the vials for 5 minutes. 18:10 Left vials to equilibriate over night. H3C-OH @ -10ºC, CHCl 3 @ -10ºC**[First you are not using equipment that will permit thermostating at exactly -10C overnight and second we are only interesting in room temp measurements for now (19-27C) JCB]**